Zebrafish larvae as a model to decision-making processes – a case study

Zebrafish decision making draw

We make thousands of decisions every day. It has been estimated that we make around 220 decisions each day only related to food (1). Choice is fundamental for our lives, as well as for the behaviour of all the other animals. Making a decision requires to integrate physiological and psychological information to choose a course […]

Precise spatiotemporal genome editing in Zebrafish using CRISPR/Cas9

Since 1901, Nobel Prizes have been awarded to “those who, during the preceding year, have conferred the greatest benefit to humankind”, in Alfred Nobel’s words1. Last year, Emmanuelle Charpentier and Jennifer Doudna were awarded with this recognized prize in the field of chemistry for their contributions to the genetic engineering tool known as CRISPR/Cas92. CRISPR/Cas9 […]

Zebrafish as a model to study the toxicity of different pesticides – a case study

Since 1974, World Environment Day has been celebrated every year on 5 June, so this month we want to acknowledge this event by reviewing a scientific article where zebrafish are used to test the toxicity of certain pesticides: Toxicity of Deltamethrin to Zebrafish Gonads Revealed by Cellular Biomarkers 1, published in Journal of Marine Science […]

Studying breast cancer with zebrafish: a case study

In this new series of articles, we will review some of the key scientific publications of 2020 which included zebrafish in their studies. This first review will cover an article published in Clinical Cancer Research on March 15th 2020: Deubiquitinase Activity Profiling Identifies UCHL1 as a Candidate Oncoprotein that Promotes TGFβ-induced breast cancer metastasis1. In […]

Small model organisms

model organisms

Over the past years, zebrafish have proven to be excellent model systems for a wide panel of experiments. We have discussed throughout our previous articles their advantages and disadvantages, how they could be incorporated to various research fields and different automation techniques which facilitate their handling in laboratories. Today we will discuss 4 other model […]

Studying zebrafish behaviour: robotics and automation

In today’s article we will take a step back from zebrafish embryos and discuss how adult zebrafish collective behaviours can be studied using robotic systems. Danio rerio, commonly known as zebrafish is a small but robust cyprinid, commonly found in food plains of the south of Asia, particularly India. They thrive in shallow and slow flowing-waters […]

Morphological and phenotypic characteristics of zebrafish embryos

zebrafish embryo expressing different fluorescent proteins

In our modern world, development of new synthetic compounds and chemicals is essential for many industries, such as the pharmaceutical, clothing and food industries. This has become a major public and environmental issue as many of these compounds can be toxic and detrimental to human and animal health. In addition to these artificial toxins, chemicals […]

Zebrafish early embryo sorting

Sorting with a pipette fertilized vs. unfertilized eggs

Over the past 20 years, the use of zebrafish in the lab has dramatically increased. In 2000, PubMed displayed 657 articles when searching for “zebrafish”. In 2020, that number shot up to 3’979 publications (1). This is most likely due to the many advantages zebrafish represent as an animal model (and which are resumed in […]