The use of animals for scientific research and experimentations started over 2,000 years ago in ancient Greece (1). Although initial experiments were purely observational and comparative with the intent to learn more about human physiology and anatomy, animals quickly became models for translational research in numerous biological fields, mostly to contribute to human and animal health.In this article, we will discuss the importance of animal experimentation for research, some animal welfare concerns, as well as laws and regulations which frame research good practices and finally how zebrafish are an interesting…